Eppendorf BioSpectrometer basic Manuale Utente Scaricare il pdf (Pagina 97)

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Evaluation procedure

Eppendorf BioSpectrometer

basic

English (EN)

12.7 Dual wavelength

For methods of the Dual Wavelength group absorbances that were measured at two wavelengths can be

calculated with each other before the calculated absorbance is evaluated further with the factor or

standard.

To determine the calculated absorbance a division or subtraction evaluation can be defined in the

parameters:

, A

= measured absorbance.

a, b, c, d = factors that are entered in the parameters. Negative figures may also be entered.

calc

u



dcAbAaA

calc

uuu

1 2 ... 92 93 94 95 96 97 98 99 100 101 102 103 104

Eppendorf BioSpectrometer 1
Table of contents 3
1 Operating instructions 7
1.1 Using this manual 7
1.3 Symbols used 8
1.4 Abbreviations used 9
Operating instructions 10
English (EN) 10
2 Product description 11
2.1 Main illustration 11
2.2 Delivery package 11
2.3 Features 12
3 Safety 13
3.1 Intended use 13
3.2 User profile 13
3.3 Warnings for intended use 13
3.3.2 Damage to device 14
 Do not open the device 16
4 Installation 17
4.1 Preparing installation 17
4.2 Selecting the location 17
4.4 Connecting the printer 17
4.4.2 Thermal printer DPU-414 18
Installation 20
5 Operation 21
5.1.1 Entering text 23
5.2 Inserting the cuvette 24
5.3.2 Measuring procedure 25
5.3.2.1 Selecting a method 25
5.3.2.2 Checking parameters 26
5.3.2.4 Measuring samples 27
5.3.2.5 Finalizing the method 27
Operation 30
6 Methods 31
6.1 Selecting a method 31
6.2.1 Absorbance method group 32
6.2.2 Routine method group 33
6.2.3 Basic method group 34
6.3 Method parameters 35
6.4 Method procedure 40
6.4.1 Check parameters 41
6.4.2 Measure standards 42
6.4.3 Measure samples 43
6.4.5 Process results 50
6.4.7 Print & export 55
7 Functions 59
7.1.1 Results memory 60
 Confirm with enter 61
7.1.4 Device settings 64
7.1.5 Device calibration 64
7.1.6 Info 65
Functions 66
8 Maintenance 67
8.1 Cleaning 67
8.3 Checking the device 69
Maintenance 70
8.4 Replacing fuses 73
9 Troubleshooting 75
9.1 General errors 75
9.2 Error messages 76
9.3 Result flags 79
Troubleshooting 82
10.1 Transport 83
10.2 Storage 83
10.3 Disposal 83
11 Technical data 85
11.1 Power supply 85
11.2 Ambient conditions 85
11.3 Weight/dimensions 85
11.4 Photometric properties 86
11.6 Application parameters 87
Technical data 88
12 Evaluation procedure 89
12.1 Absorbance values 89
12.1.3 Cuvette correction 90
12.4 Dilution 92
YYYXXXcorrXXX 93
ACFAA u 93
S, total 94
12.6.2 Calculation of the FOI 96
12.7 Dual wavelength 97
Evaluation procedure 98
13 Ordering information 99
Ordering information 100
Evaluate your manual 104

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